Journal: Heliyon

Article Title: Effect of flavonoids on antimicrobial activity of microorganisms present in dental plaque

doi: 10.1016/j.heliyon.2019.e03013

Figure Lengend Snippet: Antimicrobial effect of flavonoids in different strains of Bacteria.

Article Snippet: Actinomyces naeslundii (ATCC 12104) and Actinomyces viscosus (ATCC 15987) were grown in Tryptic Soy Agar broth containing 1 μg/mL of yeast extract, 5 μg/mL of hemin and 1 μg/mL of menadione in an anaerobic system in atmosphere of 80% N 2 , 10% CO 2 and 10% H 2 at 35 °C for 16 h. Blood Agar (DIFCO, St Louis, Mo) adding 5 mL of hemin (0.05% concentration) and 5 mL of Vitamin K stock (0.005% concentration) at anaerobic conditions and 37 °C for 48 h. Lactobacillus casei (ATCC 393) was grown in Man, Rogosa and Sharpe (MRS) broth agar (Oxoid, Basingstoke, England) and incubated at 37 °C for 16 h. Enterococcus faecalis (ATCC 8043) was grown in Trypticase Soy Broth (Becton Dickinson Microbiology Systems, Cockeysville, MD, USA), 1 μg/mL yeast extract and 15 g/L agar (Difco Laboratories, Detroit, MI, USA) and incubated at 37 °C in a reduced O 2 atmosphere.

Techniques: Bacteria